Complete Genome Mapping of Sea Buckthorn Variety

Jim Crocker
14th July, 2024

Complete Genome Mapping of Sea Buckthorn Variety

Image Source: Natural Science News, 2024

Key Findings

  • Researchers from the Chinese Academy of Sciences sequenced the genome of a hybrid sea buckthorn variety, Fructus hippophae
  • They achieved a high-quality genome assembly, covering 97.17% of the total genome sequences and validating it with a 98.80% complete BUSCO value
  • The study identified 36,475 protein-coding genes and annotated 63.68% of the genome as repetitive elements, providing a comprehensive genetic resource for future research
Sea buckthorn (Hippophae rhamnoides) is a plant known for its unique flavor and high antioxidant content, making it increasingly popular among consumers[2]. It is also valued for its nutritional and medicinal properties[3]. Despite these benefits, the cellular mechanisms behind sea buckthorn fruit development have remained largely unclear. Recently, a study conducted by the Chinese Academy of Sciences has provided new insights into the genomic structure of a hybrid variety of sea buckthorn, Fructus hippophae (Hippophae rhamnoides spp. mongolicaĂ—Hippophae rhamnoides sinensis)[1]. The researchers successfully sequenced the genome of Fructus hippophae, revealing a chromosome-level assembly of about 918.59 megabases (Mb) with a high scaffold N50 value of 83.65 Mb. They anchored 440 contigs, covering 97.17% of the total genome sequences, onto 12 pseudochromosomes. This high-quality assembly was validated through various assessment strategies, including a complete BUSCO value of 98.80% and a high mapping rate, indicating the robustness of the genome assembly. In this study, the team predicted 36,475 protein-coding genes using de-novo, homology, and transcriptome assembly strategies. Of these, 36,226 genes were functionally annotated, offering a comprehensive understanding of the gene functions within this hybrid variety. Additionally, the researchers identified repetitive elements occupying 63.68% of the genome and annotated 1,483,600 base pairs (bp) of non-coding RNA. These findings provide a foundational genomic resource that can be used to explore the mechanisms of sex differentiation in sea buckthorn and aid in the construction of a pan-genome for the species. The significance of this study is underscored when considering previous research on sea buckthorn. Earlier studies have highlighted the diverse morphological and biochemical properties of different Hippophae species[2][3]. For instance, the development patterns and cellular regulation mechanisms of sea buckthorn fruits were studied in three species, revealing differences in cell division and expansion phases[2]. Another study focused on the biochemical variations in sea buckthorn berries, such as tocopherols and tocotrienols, which vary depending on harvest date, cultivar, and environmental conditions[4]. By providing a detailed genomic map, this new study builds on the existing knowledge of sea buckthorn's morphological and biochemical diversity. It offers a genetic basis for understanding the complex traits observed in different species and cultivars. For example, the identification of protein-coding genes and non-coding RNAs can help elucidate the genetic factors that influence fruit size, shape, and biochemical composition, which were previously observed to vary significantly among different sea buckthorn species[2][4]. Moreover, the genomic information can be instrumental in addressing some of the challenges faced by sea buckthorn cultivation, such as drought, salinity, diseases, and insect pests[3]. With a better understanding of the genetic makeup, researchers can develop targeted breeding programs to enhance resistance to these stresses. The study's findings also open up possibilities for innovative biotechnological breeding methods, such as metabolite profiling and marker-assisted selection (MAS), to develop elite genotypes with specific nutritional and health-related compounds[3]. In conclusion, the chromosome-level genome assembly of Fructus hippophae provides a valuable resource for the scientific community. It not only enhances our understanding of sea buckthorn's genetic structure but also offers practical applications for improving cultivation and breeding practices. By integrating these new genomic insights with previous research findings, we can pave the way for more efficient and sustainable use of this economically and medically valuable plant.

GeneticsBiochemPlant Science

References

Main Study

1) Chromosome-level genome assembly of Hippophae rhamnoides variety.

Published 13th July, 2024

https://doi.org/10.1038/s41597-024-03549-w

Related Studies

2) The Study on Sea Buckthorn (Genus Hippophae L.) Fruit Reveals Cell Division and Cell Expansion to Promote Morphogenesis.

https://doi.org/10.3390/plants12051005

3) Advances in improvement of quality and resistance in a multipurpose crop: sea buckthorn.

https://doi.org/10.3109/07388551.2012.676024

4) Tocopherols and tocotrienols in sea buckthorn (Hippophae rhamnoides L.) berries during ripening.

https://doi.org/10.1021/jf800734v


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